Set up the CO₂ flow test

Set up and check the test here; Calibrate CO₂ flow, next, measures and adjusts the flow. A wrong regulatorDrops the cylinder's pressure (about 60 bar when full) to a working pressure, about 1 bar here. Its flathead screw fully anti-clockwise is closed. pressure found after that would mean measuring again.

1. Set the regulator to 1 bar.
2. Cap one of the two gas outlets with a luer lockA standard medical screw fitting. A male luer (the spigot) screws into a female luer (the socket). In this build, male ends mark outlets. cap.
3. Run 1/16” tubing from the open gas outlet into a water bath, under a measuring cylinder full of water.
4. Start the electroPioreactor job, or turn on the relay in the Pioreactor UI.
5. SpargeBubbling gas through the culture. Here, CO₂ from the cylinder flows down the anode on a timer set in the electroPioreactor job. until the water’s CO₂ concentration has equilibrated (assumed, or measured if you can).

Parts

• 1×CO₂ regulatorearlier step
• 1×Luer lock capearlier step
• 1×Silicone tubing, 50 cmearlier step

Check your work

1. What does the regulatorDrops the cylinder's pressure (about 60 bar when full) to a working pressure, about 1 bar here. Its flathead screw fully anti-clockwise is closed.'s outlet gauge read?

   • Well under 1 bar
   • About 1 bar
   • Well over 1 bar
   Show the answer

   Answer: About 1 bar

   • If you see Well under 1 bar: Screw the regulator in until it reads 1 bar, before touching the needle valveA fine-adjustment valve on the solenoid's front port. It sets how fast CO₂ flows; turn clockwise to close..
   • If you see Well over 1 bar: Turn the regulator anti-clockwise until it reads 1 bar, before touching the needle valve.

2. While spargingBubbling gas through the culture. Here, CO₂ from the cylinder flows down the anode on a timer set in the electroPioreactor job., where does the gas leave the vial?

   • Through both outlets: neither is capped
   • Nowhere: nothing leaves the open outlet
   • Only through the open outlet, into the measuring cylinder
   Show the answer

   Answer: Only through the open outlet, into the measuring cylinder

   • If you see Through both outlets: neither is capped: Cap one gas outlet with a luer lockA standard medical screw fitting. A male luer (the spigot) screws into a female luer (the socket). In this build, male ends mark outlets. cap, or the measuring cylinder catches only part of the flow.
   • If you see Nowhere: nothing leaves the open outlet: Check the job is sparging (or the relay is on) and the needle valveA fine-adjustment valve on the solenoid's front port. It sets how fast CO₂ flows; turn clockwise to close. is open.

≈ 0.02 g CO₂e per view