Pioreactor hardware setup

Follow Pioreactor’s 20 ml v1.1 hardware setup guide, with these changes.

1. Build it on a Raspberry Pi Zero 2 W. A Raspberry Pi 4B also works.

2. Set the vial’s own cap aside: the O-ring vial cap replaces it in Set up electrolysisSplitting water with an electric current: hydrogen at the cathode, oxygen at the anode. It supplies the hydrogen the HOB grow on..

3. Plug the stirrer into PWM channelOne of the HAT's five switched power outputs. In this build: 1 stirring, 2 media pump, 3 waste (product) pump, 4 CO₂ solenoid (relay), 5 heating. 1.

4. Connect the 12 V power supply to the HATThe Pioreactor circuit board that plugs onto the Raspberry Pi's pin header. It carries the PWM and LED outputs and the 12V barrel jack.’s barrel jack, following Pioreactor’s external power guide for your HAT.

   Before any calibration

   The peristaltic pumpsA pump that squeezes liquid along flexible tubing with rollers, so the liquid never touches the pump. and the PWM 4 CO₂ solenoidAn electric valve on PWM channel 4 that opens to sparge. It must be 3-way venting (3/2), so the line vents when it closes. need the 12 V supply. Without it the solenoid will not drive, and spargingBubbling gas through the culture. Here, CO₂ from the cylinder flows down the anode on a timer set in the electroPioreactor job. looks like a gas train fault. Setting up external power later also invalidates any stirring and pump calibration.

5. Connect the Raspberry Pi power supply to the Pi’s power socket (micro-USB on a Zero 2 W: the frontmost socket on the bottom board, with the Pioreactor logo facing you). The unit boots within about 90 seconds.

Notes

• Four or more Pioreactors on one bench can share one multi-port charger instead of one supply each: see powering a cluster.

Parts

• 1×Pioreactor 20 ml v1.1part
• 1×Raspberry Pi Zero 2 Wpart
• 1×Raspberry Pi power supplypart
• 1×Pioreactor 20 ml glass vialpart
• 1×Magnetic stir barpart
• 1×12 V power supplypart

Tools

• 1×Phillips PH0 screwdrivertool

Check your work

1. What is plugged into the HATThe Pioreactor circuit board that plugs onto the Raspberry Pi's pin header. It carries the PWM and LED outputs and the 12V barrel jack.'s barrel jack?

   • Nothing yet
   • The 12 V supply
   Show the answer

   Answer: The 12 V supply

   • If you see Nothing yet: Set it up now, before any calibration, following Pioreactor's external power guide for your HAT. Without it the pumps and the PWMOne of the HAT's five switched power outputs. In this build: 1 stirring, 2 media pump, 3 waste (product) pump, 4 CO₂ solenoid (relay), 5 heating. 4 CO₂ solenoidAn electric valve on PWM channel 4 that opens to sparge. It must be 3-way venting (3/2), so the line vents when it closes. run on the Pi's own supply, and the solenoid will not drive.

2. Which socket is the Raspberry Pi power supply in?

   • The Zero 2 W's other micro-USB, the data socket
   • The Pi's own power socket (on a Zero 2 W, the frontmost micro-USB)
   • A socket on the HAT, not the Pi
   Show the answer

   Answer: The Pi's own power socket (on a Zero 2 W, the frontmost micro-USB)

   • If you see The Zero 2 W's other micro-USB, the data socket: Move it to the frontmost socket, with the Pioreactor logo facing you: that is the Pi's power socket. The unit then boots within about 90 seconds.
   • If you see A socket on the HAT, not the Pi: The HATThe Pioreactor circuit board that plugs onto the Raspberry Pi's pin header. It carries the PWM and LED outputs and the 12V barrel jack. takes the 12 V supply. Plug the Raspberry Pi supply into the Pi's own power socket: the frontmost micro-USB on the bottom board.

≈ 0.02 g CO₂e per view